Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.

Applications:

ELISA, WB, IHC

Name of antibody:

MCM3

Immunogen:

Fusion protein of human MCM3

Full name:

minichromosome maintenance complex component 3

Synonyms：

HCC5, P1.h, RLFB, P1-MCM3

SwissProt:

P25205

ELISA Recommended dilution:

1000-5000

IHC positive control:

Human lung cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

91 kDa

WB Positive control:

K562, Jurkat, hela and 293T cells

WB Recommended dilution:

500-2000