Background:

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]

Applications:

IHC

Name of antibody:

MDC1(Phospho-Ser513)

Immunogen:

Peptide sequence around phosphorylation site of Serine 513(E-R-S(p)-Q-A) derived from Human MDC1.

Full name:

mediator of DNA-damage checkpoint 1

Synonyms：

NFBD1

SwissProt:

Q14676

IHC positive control:

Human brain tissue

IHC Recommend dilution:

50-100